Code secret : g8QX34io

Traumatic peripheral nerve injury has a sudden, debilitating effect on millions of people every year, resulting in loss of sensation and movement, significantly reducing quality of life. Fortunately, the peripheral nerves have the capacity to regenerative. The standard clinical treatment to repair peripheral nerve injuries which result in a gap in the nerve tissue is the nerve autograft. The autograft has multiple limitations, including donor site morbidity and availability, donor-recipient nerve mismatches, and poor patient satisfaction. Tissue engineering offers a promising alternative to the nerve autograft to support nerve regeneration: engineered neural tissue (EngNT) constructs comprised of aligned cells in an aligned collagen matrix. Cell source and production time can limit the clinical translatability of nerve repair constructs. Here, a novel source of endothelial cells was derived from hiPSCs, for which GMP equivalents are available, and incorporated into EngNT constructs to mimic key endothelial structures in the nerve regeneration process. The production of these EngNT constructs was also advanced to an automated technique, capable of producing nerve repair constructs in a scalable fashion.